ENTAMOEBA MOSHKOVSKII PDF

Entamoeba moshkovskii is prevalent in developing countries and morphologically indistinguishable from pathogenic Entamoeba histolytica and nonpathogenic Entamoeba dispar. It is not known if E. Mice were intracecally challenged with the trophozoites of each Entamoeba spp. In Mirpur, Dhaka, Bangladesh, E. Diarrhea occurred temporally with acquisition of a new E. In children, the acquisition of E.

Author:Nikogal Dut
Country:Turks & Caicos Islands
Language:English (Spanish)
Genre:Finance
Published (Last):28 April 2004
Pages:232
PDF File Size:12.54 Mb
ePub File Size:16.28 Mb
ISBN:488-9-84244-817-5
Downloads:87908
Price:Free* [*Free Regsitration Required]
Uploader:Kazrakazahn



Entamoeba moshkovskii is prevalent in developing countries and morphologically indistinguishable from pathogenic Entamoeba histolytica and nonpathogenic Entamoeba dispar. It is not known if E. Mice were intracecally challenged with the trophozoites of each Entamoeba spp.

In Mirpur, Dhaka, Bangladesh, E. Diarrhea occurred temporally with acquisition of a new E. In children, the acquisition of E. These data are consistent with E. Entamoeba histolytica causes extensive mortality and morbidity worldwide through diarrheal disease and abscess formation in parenchymal tissues such as liver, lung, and brain. In contrast, other amoebae that infect humans include Entamoeba dispar , Entamoeba moshkovskii, Entamoeba coli, Entamoeba hartmanni, and Endolimax nana , which have been considered nonpathogenic commensals of the human gut [ 1 — 3 ].

Dientamoeba fragilis and Entamoeba polecki have been associated with diarrhea and Entamoeba gingivalis with periodontal disease [ 4 , 5 ]. This species of Entamoeba was first identified in sewage in Moscow by Tshalaria in [ 7 ] and was initially thought to be a free-living common protozoan species in anoxic sediments and in environments such as brackish coastal pools.

The first human isolate was obtained from a resident of Laredo, Texas, who suffered from diarrhea, weight loss, and epigastric pain in [ 8 ]. At first, this isolate was named E. Both the Laredo strain and E. Subsequent molecular studies revealed that E. These studies for the most part tested stool samples submitted to clinical microbiology laboratories from patients with gastrointestinal symptoms, suggesting that E.

Thus, the ability of E. Here we tested the ability of E. In addition, we tested in a longitudinal study of children in Bangladesh not only if E. Animals were maintained under specific pathogen-free conditions at Animal Research Center for Tropical Infectious Diseases, Nagasaki University, and were challenged when they were 5—8 weeks old.

Trophozoites of the E. Trophozoites of E. Trophozoites under log phase of growth were used in the experiments. Trophozoites were harvested from culture tubes of E. Then, the trophozites were collected, and the number of trophozoites was determined. We anesthetized mice with domitor medetomidine hydrochloride: 0. Then the cecum was blotted and the peritoneum and the skin were sutured. The study was approved by the animal ethical review board of Nagasaki University. The primer sequences used for polymerase chain reaction PCR were described elsewhere [ 22 ].

The study was conducted in Mirpur, an urban slum in Dhaka. Infants were enrolled in the first week after birth and followed until one year of age, beginning in January Field research assistants FRAs visited each study house every other day and collected information related to child morbidity, especially for diarrheal illness, through a structured questionnaire.

If the FRA found any child with an acute illness, then she referred the child to the study clinic for further management by the medical officer.

Parents or guardians were also encouraged to visit the study clinic for medical assistance if the study child became sick. FRAs collected nondiarrheal monthly stool specimens as well as diarrheal stool specimens from the home or in the study field clinic. All stool specimens were transported from the field to the clinic using a cold box.

In the field clinic an aliquot of the diarrheal stool specimens was placed into Carry-Blair medium. All specimens were transported from the field clinic to the ICDDR,B Parasitology laboratory within 3 hours of collection, with a cold chain maintained. A diarrheal episode was defined as being separated from another episode by at least 3 diarrhea-free days. Informed written consent was obtained from the parents or guardians for the participation of their child in the study.

Entamoeba histolytica, Cryptosporidium, and Giardia were identified by real-time PCR as described elsewhere [ 24 ]. Multiplex RT- PCR and probe-based detection with Luminex beads for conceivable diarrhea-causative microbes was performed as described elsewhere in the literature [ 25 — 27 ]. After RT- PCR was performed with the conditions described elsewhere, samples were analyzed on the BioPlex system using bead on which coupling and hybridization were performed according to published protocols [ 28 ].

The E. Trophozoites of either E. Nonpathogenic E. These data demonstrated that in contrast to nonpathogenic E. Furthermore, obvious weight loss was observed during the course of E. Together these data indicated that E. After infection, diarrhea, colitis, and weight loss were monitored. Normal A , loose B , and bloody feces C were observed as was indicated in the results. Amoebae were observed in the lumen of the ceca in successfully infected mice D.

Macroscopic and histopathological observations of ceca in mice exhibited bloody diarrhea were shown in panels E and F. The study was repeated 3 times with similar results. The time course of each Entamoeba spp. As was reported [ 20 ], E. In contrast, E. Time course of each Entamoeba spp. The association between diarrheal episodes and infection with each Entamoeba spp. These studies were part of a prospective cohort study on diarrheal diseases [ 29 ].

Newborn children were enrolled in the Mirpur community of Dhaka, Bangladesh, and prospectively followed for diarrheal illness by every other day home visits. A total of diarrheal episodes were recorded during the first 12 months of life in children. PCR analyses of the diarrheal samples revealed that 66 episodes were positive for E. As such, in diarrheal samples, the detection rates of either E. Two episodes were found to be mixed infections with E. In order to attempt to discern if the E. The preceding 2 months of surveillance stool samples collected when the child did not have diarrhea were tested for the presence of E.

This study design therefore temporally controlled for E. In the 1 and 2 months preceding E. This supported the hypothesis that temporal acquisition of a new E. The diarrheal severity score was comparable among episodes associated with E. The duration of diarrhea was also comparable among these episodes positive for E. The mean age of the onset of diarrheal episodes associated with E.

Thus, the diarrhea related to E. As there are many microbes that can potentially induce diarrhea, the presence of other diarrheagenic microbes was tested in the 42 diarrheal samples that were associated with E. In the 42 diarrheal stool samples with E. The application of these state-of-the-art diagnostic techniques in this cohort has on average identified a minimum of 2 different enteropathogens in every diarrheal stool sample E.

Houpt and M. Taniuchi, personal communication, It was therefore not surprising that the diarrheal episodes associated with E. In order to investigate the genetic diversity in E. Twenty-six E. However, PCR did not reveal any obvious product size differences among these samples data not shown. Because same size PCR products do not necessarily mean identical DNA sequences, we sequenced PCR products directly without cloning them into any vectors in order to minimize the chances of any sequence selection to detect sequence variation.

Sequencing did reveal that the E. The sequence alignment at locus R-R revealed that the E. The SNPs detected in this study were distributed randomly across the locus R-R sequences, and as a result, these SNPs could not be used to differentiate asymptomatic and diarrheal strains of E.

The significance of this remains unknown at present. All positions are based on the consensus sequence in the alignment. This work draws into question the paradigm that E. In the murine model of intestinal amebiasis, E. In this way, E. In children in Bangladesh, the new acquisition of E. In contrast, the nonpathogenic parasite E. The finding that E. Mouse strain-dependent resistance to E.

In humans, one important means of innate resistance of intestinal epithelial cells to amebiasis is leptin, which acts via STAT3 signaling to protect intestinal epithelial cells from parasite killing [ 33 , 34 ].

In this context, it will be interesting to examine whether this observation is also true in E. If the mechanism of resistance to E.

COMMEMORATIVE BIOGRAPHICAL ENCYCLOPEDIA OF THE JUNIATA VALLEY PDF

Entamoeba moshkovskii infections in Sydney, Australia.

Entamoeba histolytica , E. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia.

JACQUES RANCIERE EL MAESTRO IGNORANTE PDF

We'd like to understand how you use our websites in order to improve them. Register your interest. Amebiasis infection is caused by Entamoeba histolytica. The nonpathogenic Entamoeba species, E. Entamoeba coinfection was found in 0. Coproscopy had a limited diagnostic performance for the diagnosis of E.

Related Articles